Evaluation of an Autoantibody
In three videos Sue Johnson demonstrates the evaluation of a patient with a warm-reactive autoantibody including:
• The Direct Antiglobulin Test (22 min)
• Preparing and Testing an Eluate (25 min)
• Performing an Autoadsorption (30 min)
Again, details of proper technique are demonstrated. The videos were made at the Blood Center of Wisconsin, which kindly provided the laboratory facilities. They were shot and edited by Sharon Karp of Media Monster in Chicago, and produced and directed by Dr. Jim Perkins, director of the III.
Basic Immunohematology: Testing in Tubes
In a series of seven videos Sue Johnson demonstrates basic “tube testing” techniques including:
• The Workstation (4 min)
• Preparing 3-5% RBC Suspensions (20 min)
• Grading Tube Tests (17 min)
• ABO and Rh typing (20 min)
• The Antibody Detection Test or “Antibody Screen” (28 min)
• Antibody Identification (35 min)
• Summary and Review (6 min)
Proper techniques are shown in the detail expected of a skilled serologist. The videos were made at the Blood Center of Wisconsin, which kindly provided the laboratory facilities. They were shot and edited by Sharon Karp of Media Monster in Chicago, and produced and directed by Dr. Jim Perkins, director of the III.
Introduction to Immunohematology
This lecture is the first in a sequence of three lectures intended to introduce the beginning student to immunohematology. It begins with a brief history of immunohematology, followed by a description of how blood group antibodies lead to the destruction of red blood cells and of some of the clinical consequences of hemolytic transfusion reactions. The lecture ends with an introduction to agglutination testing, the uses of anti-human globulin, and blood groups and blood group antibodies.
This lecture is the second in the three-lecture series on immunohematology for the beginning student. It expands on the discussion of agglutination testing introduced in the previous lecture and illustrates ABO and Rh typing tests. The use of anti-human globulin in the indirect and direct antiglobulin tests is then introduced, including the various ways in which these tests are used to answer immunohematologic questions. Various strategies used to increase the sensitivity of agglutination tests are then described, including the solid phase and gel test formats. Finally, there is a discussion of how these tests are combined in order to provide safe transfusions.
This lecture is the third in the three-lecture series on immunohematology for the beginning student. It discusses how the tests introduced in the previous lectures are used together in order to identify blood group antibodies as alloantibodies or autoantibodies, and to identify their specificities, if present. After listening to these lectures it is hoped that the student will be ready to try to solve the case studies.
About Our Lectures
These lectures are for the use of immunohematology students and teachers. They include lectures in Powerpoint on the principles of immunohematologic testing and video demonstrations of the corresponding techniques. The powerpoint lectures can be downloaded and viewed/played directly or saved to your computer. The videos stream directly.
As noted under the title of each Powerpoint lecture, most include the author’s actual spoken lecture with each slide. To hear the lecture with the slides you must view them on a computer with a sound card and speakers. Click on ‘Download Lecture”, and, when the download is complete, click on “Slide Show” on the upper menu bar and then click “View Show” from the pull down menu.
The Powerpoint lectures are copyrighted and must not be attributed to other authors. If you wish to use one or more slides in a lecture you give, leave the copyright symbol on the slide so that it is properly attributed to the author. Remove the associated sound file by clicking on the speaker symbol at the bottom right. To remove the timing associated with the slide copy all of the visual content to a fresh slide. If you use an entire lecture as is, or if you incorporate any of the slides into one of your lectures, please notify us; we are interested in disseminating this knowledge and would like to know that the material is being used.
The videos can be played simply by clicking on the icon. They illustrate actual immunohematologic techniques as performed by an expert. Although these may not replace hands-on teaching by an experienced immunohematologist, it is our hope that a dedicated student with the proper equipment could teach her- or himself to perform the procedures presented elsewhere on this website.