Posted under this tab are lectures on immunohematology and transfusion medicine in Microsoft Powerpoint and video format. The videos and dictated slide presentations need to be viewed on a computer with a sound card and speakers. Lectures will be added as available.
In a series of seven videos Sue Johnson demonstrates basic “tube testing” techniques including:
Proper techniques are shown in the detail expected of a skilled serologist. The videos were made at the Blood Center of Wisconsin, which kindly provided the laboratory facilities. They were shot and edited by Sharon Karp of Media Monster in Chicago, and produced and directed by Dr. Jim Perkins, director of the III.
I The Workstation
II Making a 3-5% RBC Suspension
III Grading Tube Test Reactions
IV ABO and Rh Typing
V The Antibody Detection Test
VI Antibody Identification
VII Summary & Review
In three videos Sue Johnson demonstrates the evaluation of a patient with a warm-reactive autoantibody including:
Again, details of proper technique are demonstrated. The videos were made at the Blood Center of Wisconsin, which kindly provided the laboratory facilities. They were shot and edited by Sharon Karp of Media Monster in Chicago, and produced and directed by Dr. Jim Perkins, director of the III.
I The Direct Antiglobulin Test
II Preparing & Testing an Eluate
III Performing an Autoadsorption
This lecture is the first in a sequence of three lectures intended to introduce the beginning student to immunohematology. It begins with a brief history of immunohematology, followed by a description of how blood group antibodies lead to the destruction of red blood cells and of some of the clinical consequences of hemolytic transfusion reactions. The lecture ends with an introduction to agglutination testing, the uses of anti-human globulin, and blood groups and blood group antibodies.
This lecture is the second in the three-lecture series on immunohematology for the beginning student. It expands on the discussion of agglutination testing introduced in the previous lecture and illustrates ABO and Rh typing tests. The use of anti-human globulin in the indirect and direct antiglobulin tests is then introduced, including the various ways in which these tests are used to answer immunohematologic questions. Various strategies used to increase the sensitivity of agglutination tests are then described, including the solid phase and gel test formats. Finally, there is a discussion of how these tests are combined in order to provide safe transfusions.
This lecture is the third in the three-lecture series on immunohematology for the beginning student. It discusses how the tests introduced in the previous lectures are used together in order to identify blood group antibodies as alloantibodies or autoantibodies, and to identify their specificities, if present. After listening to these lectures it is hoped that the student will be ready to try to solve the case studies.